XENLA GeneModel2012

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Contents

Data summary

Taira201203_XENLA_tissue

Collect total RNA from14 Tissue of Xenopus laevis J strain.

  • Brain, eye, heart, intestine, kidney, liver, lung, muscle, ovary, pancreas, skin, spleen, stomach, testis
  • Sons & daughters of single pair of frogs (Their mother frog was used for 1st BAC-end sequencing)
  • Standard Illumina sample prep. (poly-A capture)
  • Illumina HiSeq 2000, 2x100 bp
  • 108.5 billions of nucleotide calls in total.
  • 55M ~ 130M reads/tissue (27M ~ 65M pairs)

Taira201203_XENLA_stage

Collect total RNA from 11 different developmental stages of Xenopus laevis J strain embryo.

  • Stage 01, 08, 09, 10.5, 12, 15, 20, 25, 30, 35, 40
  • Sons & daughters of single pair of frogs (their mother frog was used for 1st BAC-end sequencing)
  • Standard Illumina sample prep. (poly-A capture)
  • Illumina HiSeq 2000, 2x100 bp
  • 163.8 billions of nucleotide calls in total.
  • 40M ~ 110M reads/tissue (20M ~ 55M pairs)

Assembled transcripts

Original sequences

  • From tissue samples Taira201203_XENLA_tissue_pep_final.fa Taira201203_XENLA_tissue_cdna_final.fa
  • From stage samples: Taira201203_XENLA_stage_pep_final.fa Taira201203_XENLA_stage_cdna_final.fa


Contributors

  • Masanori Taira (Graduate School of Science, University of Tokyo)
  • Shuji Takahashi (Komaba Organization for Educational Excellence, College of Arts and Sciences, University of Tokyo)
  • Toshiaki Tanaka (Tokyo Institute of Technology)
  • Atsushi Toyoda and Asao Fujiyama (National Institute of Genetics)
  • Yutaka Suzuki (Graduate School of Frontier Sciences, University of Tokyo)